Dataset: Broadbalk soil metagenomic study

Citation:  Andy Neal (2024). Dataset: Broadbalk soil metagenomic study European Nucleotide Archive https://www.ebi.ac.uk/ena/browser/view/PRJEB54688.
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Summary

Metagenomic sequence data from soil from the Broadbalk Wheat Experiment allows us to investigate the influence of inorganic fertilizer and organic amendments in the form of composted farmyard manure (FYM) on nitrogen losses and accumulation on a unique 170-year old field experiment. Also included is soil from the nearby Broadbalk Wilderness Experiment (naturally regenerating woodland since 1882) and from permanent grassland since 1838 from the Highfield Ley-Arable Experiment.

Methods

Shotgun metagenomic sequence data from the Broadbalk wheat experiment, the Broadbalk Wilderness experiment and the Highfield Ley-Arable experiment has been deposited with the European Nucleotide Archive. Three pseudo-replicate samples of soils from the grassland, woodland, FYM, 144NPK, 192NK and PK treatments were sampled in October 2015.

Treatments

  1. FYM: 35 t ha -1 of farmyard manure each year since 1843
  2. PK: P, K and Mg fertilizer since 1852 (no N fertilizer)
  3. 144NPK: 144 kgN ha-1 plus P, K and Mg fertilizer since 1852
  4. 192NK: 192 kgN ha-1 since 2001; 96kg N ha-1 between 1906-2001; K and Mg fertilizer since 1906 (no P fertilizer)
  5. Grassland: continuous grassland since 1838 (Highfield ley-arable experiment)
  6. Woodland: Naturally regenerating woodland since 1882 on the Broadbalk Wilderness experiment

Treatments 1-4 from Section 1 (continuous wheat) of the Broadbalk wheat experiment. See Neal et al (2022) for more details.

Technical Information

Shotgun metagenomic sequencing of DNA was performed using 150-base paired-end chemistry on an Illumina HiSeq 2500 sequencing platform by Beijing Novogene Bioinformatics Technology.

Related Documents

Contributors

  • Andy Neal: Researcher

Table Of Contents

  • The dataset Broadbalk soil metagenomic study is a dataset hosted on another site and eRA is maintaining this landing page. e-RA is part of the Rothamsted Long-Term Experiments - National Bioscience Research Infrastructure (RLTE-NBRI), which also covers maintenance of the Long-Term Experiments, the Rothamsted Sample Archive and Rothamsted's environmental monitoring activities including the weather stations and its role in the UK Environmental Change Network
  • The RLTE-NBRI is funded by UK Research and Innovation - Biotechnology and Biological Sciences Research Council (UKRI-BBSRC) under award BBS/E/RH/23NB0007 (2023-2028). The RLTE-NBRI is also supported by the Lawes Agricultural Trust. e-RA has been part of a National Capability since 2012, previous awards from the BBSRC were Grants BBS/E/C/00005189 (2012-2017) and BBS/E/C/000J0300 (2017-2022)

Additional Funding sources

This project received specific funding from the following sources

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For further information and assistance, please contact the e-RA curators, Sarah Perryman and Margaret Glendining using the e-RA email address: era@rothamsted.ac.uk